Chestnut Growers of America

FOLIAR ANALYSIS

 

 

PLANNING THE PROJECT

STANDARDS FOR TRACE ELEMENTS CURRENTLY UNKNOWN

During the June 17, 2000 Field Day the issue of foliar analysis was discussed. Anthony Boutard pointed out that we don’t know the base levels of trace elements required for a good producing tree. Without standards we can be applying too much or too little fertilizer and will not know it. Too much fertilizer may cause too much vegetative growth at the expense of nut production, and too little fertilizer may cause lower production levels of lower quality nuts.

There was consensus agreement that we should all participate in a leaf analysis in August this year on a voluntary basis. Anthony Boutard agreed to compile the data and provide results to us.

Leaf analysis has been used for many years as a means of determining nutrient deficiencies and as a way to establish fertilizer requirements.

OBTAINING THE TEST KITS

Leaf sample kits can be obtained from the OSU Central Analytical Laboratory at 541-737-2187, or by writing to them at:

Central Analytical Laboratory
Dept. of Crop and Soil Science
Oregon State University
Corvallis, OR 97331-2911

Leaves should be collected in mid-August and sent immediately to OSU. A single sample should represent an area no larger than 5 acres and should be from a single tree. Mark or map each tree that you sample.  

USE HEALTHY GOOD-PRODUCING TREES FOR YOUR SAMPLE

It was agreed that we want to sample trees that are producing well, and avoid those trees that show visible signs of stress or disease, or those which for whatever reason just don’t produce well. The goal here is to identify the element levels that contribute to the healthy tree.

We will be looking at the macro nutrients (N, P, K, Ca, S), as well as trace elements or micro nutrients, to develop a good picture of the healthy chestnut leaf.

Collect 10 leaves per tree, but only one per branch. Collect leaves that are free of any disease or other damage. Remove the leaves so that the petiole (leaf stem) remains on the leaf. All leaves should be picked from the middle of the current season’s terminal shoots of about average vigor.

If your samples are contaminated with soil, spray, or other visible residues, wash them in a detergent solution and rinse with soft water. Wash them quickly (one minute or less) while leaves are still fresh, to avoid loss of nutrient elements. Remove excess moisture by blotting with a paper towel. Then allow the leaves to air dry.

Fill out the sample kit’s information sheet completely -- all this information may be useful. Put the sheet, along with the dried leaves, into the paper bag furnished in the kit.  Seal the bag securely and mail it to the lab at the address given previously. No other container is necessary.

If you are submitting more than one sample at a time make sure to code them in some way that they can be identified by you when the results are returned.

Samples will be analyzed for nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, boron, iron, manganese, zinc, and copper.  The lab will send a computer-printout to you with the results.  Make a copy and send it to Anthony Boutard.


FOLIAR ANALYSIS PROJECT PROVIDES BASELINE FOR "NORMAL" IN WESTERN ORCHARDS
By Anthony Boutard
as published in The Western Chestnut, Fall 2000

Analyzing the nutrient levels in the foliage of crop plants is a useful tool for determining fertilizing needs and application efficacy, and assessing the general health of the chestnut orchard.  In order to make sense of the foliar analysis, we must know the normal state of affairs in a healthy chestnut leaf.  

For many crop plants grown in the western U.S., reliable tables of foliar nutrient content ranges have been established.  Because chestnuts are a minor crop, the information has not been developed for the species in this region.  There might be a temptation to use tables from another nut crop.  However, neither walnuts nor hazelnuts are closely related to chestnuts; soil preferences are different, and the foliage of those trees is different in chemical composition.  There are some sources addressing foliar nutrient levels in chestnuts, however they were generated in different soils and climates, and we need local analysis to refine that information.             

During this summer, several chestnut growers volunteered to undertake an analysis of some of their healthiest trees to help generate local nutrient level ranges for the west coast.  Leaf samples were collected between the 8th and 24th of August.  All of the foliage samples were taken from young orchard grown trees (less than 15 years).  

The first of the OSU reports we received did not include any recommendations concerning nutrient levels.  Subsequent reports did because it was possible to observe patterns in nutrient levels once the number of samples increased.  According to Nancy Kyle at the Central Analytical Laboratory, 11 samples were received in August, and two in September.  As of writing this article, only six of the samples analyzed at OSU have been sent to me. I received four more prepared by Agri-Check, and two were prepared by Californian labs. 

As an interim measure, the Central Analytical Laboratory has adopted hazelnut recommendations for chestnuts.  Approach these preliminary recommendations carefully.  For example, under potassium (K), some of the reports noted a deficiency and suggested that adding lime may free up potassium in the soil.  It is important to remember that the optimal pH range for chestnuts is between 5.0 and 6.0 (Crawford 1995: Bourgeois 1992, 252), and liming is not desirable if the pH is shifted out of this range.  According to Breisch (1995, 155), chlorosis in chestnuts can result from an elevated pH, which blocks the absorption of iron and other nutrients.  For both conventional and organic orchards, banding K-Mag at the drip line is an effective method of increasing available potassium without increasing the soil pH. 

Breisch (1995, 156 & 159) also stresses the importance of boron and manganese for tree health and nut production.  When boron is deficient, young nut bearing flowers of the Japanese chestnut (Castanea crenata) have been observed falling from the tree just after flowering.  In many cane fruits and nuts, boron deficiencies are also associated with fruiting problems.  Boron has a role in the synthesis of the bases that form DNA and RNA, and the movement of sugars in the phloem.  (Salisbury and Ross 1978, 92)   Breisch notes that chestnuts are heavy consumers of manganese, which tends to be abundant in acidic soils.  Shattuck (1991) notes that chestnuts tend to show signs of manganese deficiency in soils where the pH exceeds 6.5.  Leaf tissue concentrations below 41 ppm were identified as clearly deficient.  However Shattuck also observed deficiency symptoms at higher levels (55 to 100 ppm), though other deficiencies, nitrogen and iron, made it difficult to clearly interpret the results.  All of the reports we received showed manganese levels in good shape.   

Table 1 below provides the raw results of the individual tests in percent or parts per million dry weight.  Table 2 reports various published dry weight composition ranges from other regions.  Table 3 is a statistical summary of the data in Table 1.  I have confined summary in Table 3 to the Washington and Oregon results.  We have only two reports from California, and those orchards are growing in unique situations.  Those samples were collected and tested in 1999.

At this point, we can ponder the range of values collected, and evaluate them against published levels reported in Table 2.  The next formal step in this project falls to Jeff Olsen, OSU Extension for nuts, who will take the results of the effort and create a range from deficient to excess for the various nutrients, and then formulate chestnut specific recommendations for addressing deficiencies. 

I want to thank those who took the time to sample their trees and send me the analysis results.  I am very also grateful to Sandra Anagnostakis for her quick offer of the Shattuck and Miller papers, which are helpful.

 References:

 Bourgeois, C.  1992.  Le Chataignier:  Un Arbre, Une Bois.  Paris:  Institute Pour Le Developpement Forestier.  367 p. 

Breisch, H.  1995.  Châtaignes et Marrons.  Paris: Center technique interprofessionnel des fruites et légumes (Ctifl).  239 p.

 Crawford, M.  1995.  Chestnuts: Production and Culture.  Darlington, UK: Agroforstry Research Trust.  52 p.

 Miller, G.  undated.  Chestnut Information Sheet.  Empire Chestnut Company, Carrollton, Ohio.

 Ridley, D. and J Beaumont. 1999.  The Australian Chestnut Growers' Resource Manual.  Myrtleford, Australia: Department of Natural Resources and Environment.  Page 5.2.

 Salisbury, F.B. and C.W. Ross.  1978  Plant Physiology, 2nd edition.  Belmont, California: Wadsworth.  422 p.

 Shattuck, V. I.  1991.   Manganese levels in Ontario-grown chestnuts.  Annual       Report Northern Nut Growers Association  82: 99-102.

 

 Bo

Table 1.  Foliar nutrient content for western chestnut trees in terms of percent (%) or parts per million (ppm) of dry weight.

Sample

N

%

S 2

%

P

%

K

%

Ca

%

Mg

%

B

ppm

Zn

ppm

Mn

ppm

Cu

ppm

Fe 2

ppm

soil

pH

soil type

Portland, OR1

2.57

0.20

0.20

0.92

0.79

0.25

73

29

462

9

105

5.6

silt loam

Gaston, OR1

2.72

0.23

0.23

1.02

1.00

0.22

63

70

675

10

228

5.7

silt loam

Gaston, OR1

2.68

0.19

0.30

1.01

1.20

0.28

108

70

1010

9

186

5.7

silt loam

Moses Lake, WA

2.52

 

0.23

0.57

1.75

0.62

45

28

316

6

 

 

 

Moses Lake, WA

2.52

 

0.31

0.70

1.46

0.62

41

36

260

6

 

 

 

Monroe, OR

2.71

 

0.16

0.75

1.29

0.52

69

33

382

4

 

 

 

Iselton, CA

3.55

 

0.25

0.77

1.88

0.52

299

50

253

10

91

7.1

silty clay loam

Ridgefield, WA

2.74

 

0.34

0.63

1.65

0.43

109

51

220

10

 

5.6

 

Lebanon, OR

2.15

 

0.20

0.67

1.19

0.37

126

33

144

7

 

 

 

Lebanon, OR

2.06

 

0.17

0.60

1.22

0.40

137

28

137

7

 

 

 

Mossyrock, WA

2.27

0.14

0.15

0.95

0.88

0.18

31

29

111

8

91

 

volcanic loam

Visalia, CA

2.47

 

0.21

0.42

2.16

0.32

38

280

120

6

777

 

volcanic loam

Notes:

1.         The first three samples are from certified organic farms.  
2.         The Central Analytical Laboratory at OSU does not include sulfur or iron in its analysis, hence the blank spaces.

 Table 2.  Published descriptions of foliar nutrient levels in terms of percent (%) or parts per million (ppm) of dry weight.

Reference

N

%

S

%

P

%

K

%

Ca

%

Mg

%

B

ppm

Zn

ppm

Mn

ppm

Cu

ppm

Fe

ppm

region

Breisch 1995

Table VI - 1

1.80 - 2.50

 

0.30 - 0.40

0.6   -  1.0

0.8  - 1.20

0.20   - 0.40

40   -   50

25   -   35

300  - 1000

10   -   15

60   -  100

France

Miller  - Sample of Field Trees

2.00 - 3.00

0.14 - 0.16 

0.12 - 0.20

0.50 - 0.80

0.70 - 1.00

0.25 - 0.35

30   -   80

20   -   50

170  -  300

4     -     8

45   -  100

Eastern US

Shattuck 1991

 

 

 

 

 

 

 

 

126  -  700

 

 

Ontario, Canada

Ridley and Beaumont 1999

2.40 - 2.90

0.15 - 0.25

0.14 - 0.30

0.80 - 1.60

0.60 - 1.40

0.25 - 0.70

33   -   90

9     -   68

50   -  700

4     -   20

9     -   68

Australia

 Table 3.  Statistical summary of the Oregon and Washington results

 

N

%

S

%

P

%

K

%

Ca

%

Mg

%

B

ppm

Zn

ppm

Mn

ppm

Cu

ppm

Fe

ppm

Median

2.55

--

0.22

0.73

1.21

0.39

71

33

288

8

--

Mean

2.49

--

0.23

0.78

1.24

0.39

80

41

372

8

--

High

2.74

0.23

0.34

1.02

1.75

0.62

137

70

1010

10

777

Low

2.06

0.14

0.15

0.57

0.79

0.20

31

28

111

4

91

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